Let the 2015 Dubsmash Wars for Charity begin! "Agent Carter" stars Hayley Atwell and James D'Arcy kicked things off on Wednesday with a performance of Beyoncé's "Single Ladies" in the first round of Dubsmash Wars for Charity against their Marvel TV foes Clark Gregg and Chloe Bennet from "Agents of S.H.I.E.L.D."

Atwell and D'Arcy received a little help from the newly single "Muppets" prima donna Miss Piggy. They all wore Peggy Carter's signature blue suit and red fedora as they threw their hands up to the female anthem.

Watch the video here.

Fans can now vote for the #TeamCarter video by donating $1, which counts as one vote cast, through the fundraising website CrowdRise. Money donated toward their videos will benefit the organization "Stomp Out Bullying!" while #TeamSHIELD will compete for St. Jude Children's Research Hospital. They will put out their first video on Sept. 9.

The Dubsmash rivalry between the ABC shows' casts started in July at San Diego Comic-Con. They then decided to continue the battle, but for a good cause.

"Recently James and I got into a musical tussle with some very dear friends," Atwell said in the video announcement for the "Dubsmash Wars for Charity" last month. "But to our surprise," D'Arcy joined in before handing it back over to Atwell, "that innocent Comic-Con skirmish took on a life of its own."

The Dubsmash app allows users to lip-sync their favorite songs or quotes in short, 10-second videos. Gregg and Bennet started things off with their versions of "It Takes Two" by Rob Base and DJ EZ Rock followed by Rihanna's "B---h Better Have My Money."

Atwell and D'Arcy then dropped the mic and threw it "out of the f***ing window" when they recruited Captain America himself, Chris Evans, to join them in videos "singing" Queen's "Another One Bites the Dust" and "(I've Had) The Time of My Life."

Be sure to stay tuned with HNGN when the Dubsmash Wars 2015 continue next Wednesday.